Table 1.
Antibodies.
| Antigen | Clone | Label | Manufacturer |
|---|---|---|---|
| MHC-II | M5/114.15.2 | FITC | eBioscience |
| CD11c | HL3 | PE | BD Biosciences |
| Sirp-α | P84 | PerCP-eFluor710 | eBioscience |
| CD19 | 1D3 | PE-Cy7 | BD Biosciences |
| CD8α | 53–6.7 | APC | BD Biosciences |
| CD11b | M1/70 | APC-Cy7 | BD Biosciences |
| CD24 | M1/69 | eFluor450 | eBioscience |
| CD86 | GL-1 | PerCP | BioLegend |
| F4/80 | BM8 | APC | eBioscience |
| Ly6C | HK1.4 | eFluor450 | eBioscience |
| CD4 | RM4-5 | AF488 | BD Pharmingen |
| CD62L | MEL-14 | PE | eBioscience |
| CD335 | 29A1.4 | PerCP-Cy5.5 | BD Biosciences |
| CD44 | IM7 | PE-Cy7 | BD Biosciences |
| CD3e | 145-2C11 | APC-Cy7 | BD Pharmingen |
| CD25 | eBio3C7 | eFluor450 | eBioscience |
Antibodies used for flow cytometry. All antibodies were diluted 1000x in flow cytometry buffer prior to use (CD19 and CD335 were used in a 500x dilution).