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. 2021 Jul 19;12(1):274–290. doi: 10.1016/j.apsb.2021.07.009

Figure 6.

Figure 6

Western blotting analysis of KRAS downstream signal pathways. (A) Phosphorylation levels of t-ERK and t-AKT by stimulated KRAS-dependent Mia PaCa-2 cells with EGF (125 ng/mL, 10 min). From top to bottom: phosphorylated ERK on Thr202 and Tyr204 (p-ERK), total level of ERK (t-ERK), phosphorylated AKT on S473 (p-AKT), total level of AKT (t-AKT), and loading control (GAPDH). (B and C) Gray intensity analysis of the blots in quantification of p-ERK/t-ERK ± SEM (top) and p-AKT/t-AKT ± SEM (bottom) was standardized to the EGF-stimulated control (1% DMSO). Data were represented as means ± SEM, n = 3, ∗∗∗P < 0.001.