Figure 7. Relative abundance of recombinant human LDH-A tetramer, dimer, and monomer as a function of pH and enzyme concentration.

DLS measurements performed at pH 7.5 (A–C), 6.5 (D–F), and 5.0 (G–I) in the presence of 8.4 (A,D,G), 4.2 (B,E,H), or 2.1 (C,F,I) µM tetrameric enzyme (33.6, 16.8, and 8.4 µM subunits, respectively). The distributions of the observed scattering intensities (empty circles, average of three measurements) were deconvoluted with the Fityk software into an ensemble of Gaussian components (blue, red, and pink lines). The fittings to the experimental observations accordingly obtained are reported with green lines.