Table 1. Kinetic parameters of recombinant and natural human LDH.
| pH | K0.5 (μM) | Vmax (nM/s) | Hill |
|---|---|---|---|
| Human LDH-A, recombinant | |||
| 5.0 | 24.7 ± 3.1 | 4.1 ± 0.3 | 1.78 ± 0.27 |
| 5.5 | 43.4 ± 8.7 | 12.5 ± 2.2 | 2.32 ± 0.49 |
| 6.0 | 15.7 ± 1.0 | 24.2 ± 0.8 | 1.73 ± 0.18 |
| 6.5 | 14.9 ± 0.6 | 41 ± 1 | 2.12 ± 0.18 |
| 7.0 | 34.7 ± 1.2 | 72 ± 1 | 1.34 ± 0.05 |
| 7.5 | 252 ± 30 | 276 ± 16 | - |
| 8.0 | 500 ± 44 | 596 ± 33 | - |
| Human LDH-A, natural | |||
| 5.0 | 56 ± 4 | 19 ± 1 | 2.01 ± 0.28 |
| 5.5 | 34 ± 3 | 87 ± 4 | 1.65 ± 0.19 |
| 6.0 | 43 ± 4 | 82 ± 4 | 1.51 ± 0.21 |
| 6.5 | 82 ± 11 | 211 ± 14 | 1.13 ± 0.08 |
| 7.0 | 95 ± 9 | 296 ± 11 | - |
| 7.5 | 210 ± 17 | 389 ± 15 | - |
| 8.0 | 1083 ± 359 | 1306 ± 330 | - |