Figure 7.

Depletion of DTL suppresses BCa cell proliferation and restrains G2 cell cycle transition. (a) qRT-PCR analysis assessed knockdown efficiency of three DTL siRNAs in UMUC3 and T24 cells, and siRNA1 represents the highest knockdown efficiency. Therefore, siRNA1 was chosen to be used in subsequent experiments. (b) Immunofluorescence assessed knockdown efficiency of siRNA1 in UMUC3 and T24 cells. (c) MTT assays in UMUC3 and T24 cells represented cell viability after depletion of DTL. (d) Clone formation assay represented cell proliferation viability after depletion of DTL, and (e) the clone formation cell counts were statistically analyzed. (f) Flow cytometry represented the alteration of the cell cycle after depletion of DTL in UMUC3 and T24 cells, and statistical significance was assessed using two-tailed t-tests. (g) Western blotting analysis represented the alteration of cycle-associated protein. ^∗p < 0.01, ^∗∗p < 0.001.