Figure 9.

Reduction of DTL suppresses BCa growth in vivo. (a) The green fluorescence of UMUC3 stable cells. (b, c) qRT-PCR and western blotting analysis verified DTL depletion efficiency in UMUC3 stable cells. (d) Xenograft mouse models were established by subcutaneously injecting LV-NC cells or LV-shDTL cells and monitored continuously for 5 weeks; then, the mice were sacrificed and the tumors were dissected. (e, f) Statistical analysis of tumor volume and weight in two groups. (g) HE staining assessed the nucleus atypia of tumors, and IHC staining detected the expression of Ki67. (h) Immunofluorescence staining detected the expression of DTL and Ki67. ^∗p < 0.01, ^∗∗p < 0.001.