Figure 1.
Characterization of DM NGs. (A) Hydrodynamic diameter (Dh) and TEM image of DM NGs. (B) The change in hydrodynamic diameter and zeta potential of DM NGs in 15 days. (C) Zeta potentials of MAN NGs, DOX NGs and DM NGs. (D) UV–Vis spectra and fluorescence spectra of Free DOX and DM NGs. (E) The controlled release behavior of DM NGs after GSH treatment. (F) In vitro release pattern of DOX from DM NGs. (G) Flow cytometric profile of DM NGs. (H) CLSM images of lysosomal colocalization. (I) Quantification of intracellular DOX in 4T1 cells for 1 and 2 h. (J) GSH level was stained by Thiol-Tracker Violet (green). (K) The quantification of intracellular GSH in 4T1 cells. Data are presented as mean ± SD (n = 3). ∗P < 0.05; ∗∗P < 0.01.