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. 2021 May 21;12(1):451–466. doi: 10.1016/j.apsb.2021.05.016

Figure 2.

Figure 2

Cytotoxicity and ICD studies of DM NGs in vitro. (A) Cytotoxicity of Free DOX, free MAN, MAN NGs, DOX NGs and DM NGs in 4T1 cells for 24 h. (B) Fluorescence images of 4T1 cells after treatments and FDA staining. (C) Area occupied by cells during live staining. (D) and (E) Scratch-wound healing assay. CLSM examinations of HMGB1 (F) and CRT (H) release. Fluorescence intensity of HMGB1 (G) and CRT (I) expressed. Data are presented as mean ± SD (n = 3). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.