Table 2.
Subtypes of CD45+ immune cells resided in intestinal epithelium and lamina propria of WT and D6KO mice
| Subtype | Cell counts |
% of CD45+ cell |
% of main type |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | D6KO | WT (%) | D6KO (%) | Deviation (%) | Assessmenta | Odds ratio | p-value | WT (%) | D6KO (%)< | Deviation (%) | Assessmenta | Odds ratio | p-value | |
| Tgd (3176 cells in WT; and 3132 cells in D6KO) | ||||||||||||||
| Tgd.VG5- | 291 | 551 | 7.74 | 15.05 | 7.32 | ↑↑ | 2.11 | 0.0000 | 9.16 | 17.59 | 8.43 | ↑↑ | 2.12 | 0.0000 |
| Tgd.VG5+ | 1530 | 1327 | 40.69 | 36.26 | -4.43 | ↓↓ | 0.83 | 0.0001 | 48.17 | 42.37 | -5.80 | ↓↓ | 0.79 | 0.0000 |
| Tgd.VG5+.ACT | 1334 | 1230 | 35.48 | 33.61 | -1.87 | ↓ | 0.92 | 0.0921 | 42.00 | 39.27 | -2.73 | ↓↓ | 0.89 | 0.0275 |
| Tgd.VG2+ | 16 | 22 | 0.43 | 0.60 | 0.18 | ↑ | 1.42 | 0.3306 | 0.50 | 0.70 | 0.20 | ↑ | 1.40 | 0.3324 |
| Tgd.mat.VG2+ | 4 | 1 | 0.11 | 0.03 | -0.08 | = | 0.26 | 0.3751 | 0.13 | 0.03 | -0.09 | = | 0.25 | 0.3749 |
| Tgd.imm.VG1+ | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4965 |
| Tgd.mat.vg3 | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 |
| CD8+T cell (124 cells in WT; and 108 cells in D6KO) | ||||||||||||||
| T.8NVE.OT1 | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 0.81 | 0.00 | -0.81 | ND | 0.00 | 1.0000 |
| T.8EFF.OT1.12HR.LISOVA | 62 | 34 | 1.65 | 0.93 | -0.72 | ↓↓ | 0.56 | 0.0073 | 50.00 | 31.48 | -18.52 | ↓↓ | 0.46 | 0.0050 |
| T.8EFF.OT1.24HR.LISOVA | 12 | 9 | 0.32 | 0.25 | -0.07 | = | 0.77 | 0.6638 | 9.68 | 8.33 | -1.34 | ↓ | 0.85 | 0.8203 |
| T.8EFF.OT1.48HR.LISOVA | 4 | 2 | 0.11 | 0.05 | -0.05 | = | 0.51 | 0.6875 | 3.23 | 1.85 | -1.37 | ↓ | 0.57 | 0.6880 |
| T.8EFF.OT1.D10LIS | 7 | 17 | 0.19 | 0.46 | 0.28 | ↑↑ | 2.50 | 0.0406 | 5.65 | 15.74 | 10.10 | ↑↑ | 3.12 | 0.0163 |
| T.8MEM.OT1.D45.LISOVA | 28 | 34 | 0.74 | 0.93 | 0.18 | ↑ | 1.25 | 0.4445 | 22.58 | 31.48 | 8.90 | ↑ | 1.58 | 0.1388 |
| T.8EFF.TBET-.OT1LISOVA | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 0.93 | 0.93 | ND | INF | 0.4655 |
| T.8Mem | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 0.93 | 0.93 | ND | INF | 0.4655 |
| T.8NVE | 1 | 1 | 0.03 | 0.03 | 0.00 | ND | 1.03 | 1.0000 | 0.81 | 0.93 | 0.12 | ND | 1.15 | 1.0000 |
| T.CD8.1H | 9 | 8 | 0.24 | 0.22 | -0.02 | = | 0.91 | 1.0000 | 7.26 | 7.41 | 0.15 | ↑ | 1.02 | 1.0000 |
| T.CD8.5H | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 0.93 | 0.93 | ND | INF | 0.4655 |
| Macrophage (93 cells in WT; and 102 cells in D6KO) | ||||||||||||||
| MF.103-11B+ | 26 | 51 | 0.69 | 1.39 | 0.70 | ↑↑ | 2.03 | 0.0028 | 27.96 | 50.00 | 22.04 | ↑↑ | 2.58 | 0.0020 |
| MF.103-11B+.SALM3 | 18 | 10 | 0.48 | 0.27 | -0.21 | ↓ | 0.57 | 0.1852 | 19.35 | 9.80 | -9.55 | ↓ | 0.45 | 0.0673 |
| MF.103-11B+24- | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 1.08 | 0.00 | -1.08 | ND | 0.00 | 0.4769 |
| MF.103CLOSER | 6 | 5 | 0.16 | 0.14 | -0.02 | = | 0.86 | 1.0000 | 6.45 | 4.90 | -1.55 | ↓ | 0.75 | 0.7598 |
| MF.11CLOSER | 17 | 14 | 0.45 | 0.38 | -0.07 | = | 0.85 | 0.7203 | 18.28 | 13.73 | -4.55 | ↓ | 0.71 | 0.4359 |
| MF.11CLOSER.SALM3 | 22 | 17 | 0.59 | 0.46 | -0.12 | ↓ | 0.79 | 0.5228 | 23.66 | 16.67 | -6.99 | ↓ | 0.65 | 0.2824 |
| MF.169+11CHI | 2 | 3 | 0.05 | 0.08 | 0.03 | = | 1.54 | 0.6833 | 2.15 | 2.94 | 0.79 | ↑ | 1.38 | 1.0000 |
| MF.II+480LO | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 0.98 | 0.98 | ND | INF | 1.0000 |
| MF.SBCAPS | 1 | 1 | 0.03 | 0.03 | 0.00 | ND | 1.03 | 1.0000 | 1.08 | 0.98 | -0.09 | ND | 0.91 | 1.0000 |
| DC (121 cells in WT; and 73 cells in D6KO) | ||||||||||||||
| DC | 10 | 5 | 0.27 | 0.14 | -0.13 | ↓ | 0.51 | 0.3019 | 8.26 | 6.85 | -1.42 | ↓ | 0.82 | 0.7888 |
| DC.103+11B- | 5 | 4 | 0.13 | 0.11 | -0.02 | = | 0.82 | 1.0000 | 4.13 | 5.48 | 1.35 | ↑ | 1.34 | 0.7310 |
| DC.103-11B+24+ | 16 | 6 | 0.43 | 0.16 | -0.26 | ↓ | 0.38 | 0.0526 | 13.22 | 8.22 | -5.00 | ↓ | 0.59 | 0.3543 |
| DC.8+ | 6 | 3 | 0.16 | 0.08 | -0.08 | = | 0.51 | 0.5079 | 4.96 | 4.11 | -0.85 | ↓ | 0.82 | 1.0000 |
| DC.8- | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 0.83 | 0.00 | -0.83 | ND | 0.00 | 1.0000 |
| DC.8-4-11B+ | 6 | 0 | 0.16 | 0.00 | -0.16 | ↓↓ | 0.00 | 0.0313 | 4.96 | 0.00 | -4.96 | ↓↓ | 0.00 | 0.0851 |
| DC.8-4-11B- | 12 | 3 | 0.32 | 0.08 | -0.24 | ↓↓ | 0.26 | 0.0352 | 9.92 | 4.11 | -5.81 | ↓↓ | 0.39 | 0.1734 |
| DC.LC | 2 | 0 | 0.05 | 0.00 | -0.05 | ND | 0.00 | 0.5000 | 1.65 | 0.00 | -1.65 | ND | 0.00 | 0.5282 |
| DC.PDC.8+ | 50 | 39 | 1.33 | 1.07 | -0.26 | ↓ | 0.80 | 0.3373 | 41.32 | 53.42 | 12.10 | ↑ | 1.63 | 0.1052 |
| DC.PDC.8- | 13 | 13 | 0.35 | 0.36 | 0.01 | = | 1.03 | 1.0000 | 10.74 | 17.81 | 7.06 | ↑ | 1.80 | 0.1933 |
| B cell (96 cells in WT; and 82 cells in D6KO) | ||||||||||||||
| B.CD19CONTROL | 6 | 8 | 0.16 | 0.22 | 0.06 | = | 1.37 | 0.6024 | 6.25 | 9.76 | 3.51 | ↑ | 1.62 | 0.4157 |
| B.FrE | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 1.04 | 0.00 | -1.04 | ND | 0.00 | 1.0000 |
| B.T3 | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 1.22 | 1.22 | ND | INF | 0.4607 |
| B.FO | 0 | 7 | 0.00 | 0.19 | 0.19 | ↑ | INF | 0.0071 | 0.00 | 8.54 | 8.54 | ↑ | INF | 0.0038 |
| B.GC | 3 | 1 | 0.08 | 0.03 | -0.05 | ND | 0.34 | 0.6250 | 3.13 | 1.22 | -1.91 | ND | 0.38 | 0.6255 |
| B.FrF | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 1.04 | 0.00 | -1.04 | ND | 0.00 | 1.0000 |
| B.MEM | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 1.22 | 1.22 | ND | INF | 0.4607 |
| B.MZ | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 1.04 | 0.00 | -1.04 | ND | 0.00 | 1.0000 |
| B1A | 84 | 64 | 2.23 | 1.75 | -0.49 | ↓ | 0.78 | 0.1361 | 87.50 | 78.05 | -9.45 | ↓ | 0.51 | 0.1099 |
| NKT (20 cells in WT; 62 cells in D6KO) | ||||||||||||||
| NKT.4+ | 11 | 27 | 0.29 | 0.74 | 0.45 | ↑↑ | 2.53 | 0.0086 | 55.00 | 43.55 | -11.45 | ↓ | 0.63 | 0.4436 |
| NKT.4- | 4 | 20 | 0.11 | 0.55 | 0.44 | ↑↑ | 5.16 | 0.0008 | 20.00 | 32.26 | 12.26 | ↑ | 1.90 | 0.4005 |
| NKT.44+NK1.1+ | 5 | 14 | 0.13 | 0.38 | 0.25 | ↑↑ | 2.88 | 0.0388 | 25.00 | 22.58 | -2.42 | ↓ | 0.88 | 1.0000 |
| NKT.44+NK1.1- | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 1.61 | 1.61 | ND | INF | 1.0000 |
| NK cell (40 cells in WT; 24 cells in D6KO) | ||||||||||||||
| LPL.NCR+CNK | 15 | 13 | 0.40 | 0.36 | -0.04 | = | 0.89 | 0.8507 | 37.50 | 54.17 | 16.67 | ↑ | 1.97 | 0.2073 |
| NK.DAP10- | 25 | 11 | 0.66 | 0.30 | -0.36 | ↓↓ | 0.45 | 0.0289 | 62.50 | 45.83 | -16.67 | ↓↓ | 0.51 | 0.2073 |
| CD4+T cell (24 cells in WT; and 31 cells in D6KO) | ||||||||||||||
| T.4EFF49D+11A+.D8.LCMV | 0 | 2 | 0.00 | 0.05 | 0.05 | ND | INF | 0.2433 | 0.00 | 6.45 | 6.45 | ND | INF | 0.4990 |
| T.4MEM44H62L | 4 | 7 | 0.11 | 0.19 | 0.08 | = | 1.80 | 0.3813 | 16.67 | 22.58 | 5.91 | ↑ | 1.46 | 0.7385 |
| T.4Mem | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 3.23 | 3.23 | ND | INF | 1.0000 |
| T.CD4.1H | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 4.17 | 0.00 | -4.17 | ND | 0.00 | 0.4364 |
| T.CD4.5H | 6 | 5 | 0.16 | 0.14 | -0.02 | = | 0.86 | 1.0000 | 25.00 | 16.13 | -8.87 | ↓ | 0.58 | 0.5049 |
| T.CD4.CTR | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 4.17 | 0.00 | -4.17 | ND | 0.00 | 0.4364 |
| T.CD4TESTCJ | 12 | 16 | 0.32 | 0.44 | 0.12 | ↑ | 1.37 | 0.4524 | 50.00 | 51.61 | 1.61 | ↑ | 1.07 | 1.0000 |
| Treg (19 cells in WT; and 21 cells in D6KO) | ||||||||||||||
| T.4FP3+25+ | 1 | 1 | 0.03 | 0.03 | 0.00 | ND | 1.03 | 1.0000 | 5.26 | 4.76 | -0.50 | ND | 0.90 | 1.0000 |
| T.Tregs | 18 | 20 | 0.48 | 0.55 | 0.07 | = | 1.14 | 0.7462 | 94.74 | 95.24 | 0.50 | ↑ | 1.11 | 1.0000 |
| ILC1 (20 cells in WT; and 13 cells in D6KO) | ||||||||||||||
| LIV.ILC1.DX5- | 4 | 1 | 0.11 | 0.03 | -0.08 | = | 0.26 | 0.3751 | 20.00 | 7.69 | -12.31 | ↓ | 0.33 | 0.6253 |
| LPL.NCR+ILC1 | 16 | 12 | 0.43 | 0.33 | -0.10 | ↓ | 0.77 | 0.5718 | 80.00 | 92.31 | 12.31 | ↑ | 3.00 | 0.6253 |
| ILC2 (10 cells in WT; and 4 cells in D6KO) | ||||||||||||||
| ILC2 | 10 | 4 | 0.27 | 0.11 | -0.16 | ↓ | 0.41 | 0.1797 | 100.00 | 100.00 | 0.00 | ND | ND | 1.0000 |
| Monocyte (6 cells in WT; and 7 cells in D6KO) | ||||||||||||||
| MO.6C+II- | 6 | 6 | 0.16 | 0.16 | 0.00 | = | 1.03 | 1.0000 | 100.00 | 85.71 | -14.29 | ↓ | 0.00 | 1.0000 |
| MO.6C-II+ | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 14.29 | 14.29 | ND | INF | 1.0000 |
| ILC3 (5 cells in WT; and 0 cell in D6KO) | ||||||||||||||
| ILC3.LTI.4+ | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 20.00 | ND | ND | ND | ND | 1.0000 |
| ILC3.LTI.CD4- | 4 | 0 | 0.11 | 0.00 | -0.11 | ND | 0.00 | 0.1250 | 80.00 | ND | ND | ND | ND | 1.0000 |
| Other T cells (2 cells in WT; and 0 cell in D6KO) | ||||||||||||||
| T.ETP | 2 | 0 | 0.05 | 0.00 | -0.05 | ND | 0.00 | 0.5000 | 100.00 | ND | ND | ND | ND | 1.0000 |
| Neutrophil (2 cells in WT; and 0 cell in D6KO) | ||||||||||||||
| GN.ARTH | 2 | 0 | 0.05 | 0.00 | -0.05% | ND | 0.00 | 0.5000 | 100.00 | ND | ND | ND | ND | 1.0000 |
| Mast cell (1 cell in WT; and 1 cell in D6KO) | ||||||||||||||
| MC.ES | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 100.00 | 0.00 | -100.00 | ND | 0.00 | 1.0000 |
| MC.TR | 0 | 1 | 0.00 | 0.03 | 0.03 | ND | INF | 0.4933 | 0.00 | 100.00 | 100.00 | ND | INF | 1.0000 |
| Basophils (1 cell in WT; and 0 cell in D6KO) | ||||||||||||||
| BA | 1 | 0 | 0.03 | 0.00 | -0.03 | ND | 0.00 | 1.0000 | 100.00 | ND | ND | ND | ND | 1.0000 |
D6KO, Dusp6 knockout; INF, infinite; ND, not determined; WT, wild-type.
See also Figure 2.
a
Assessment rules: ND, not determined due to insufficiency of sample size (total counts <5) that could be resulted from low isolation rate and/or identification limitation; =, no difference reflected by a value of Deviation between −0.1 and 0.1%; ↑, a trend of increase with no statistical significance; ↓, a trend of decrease with no statistical significance; ↑↑, significant increase; ↓↓, significant decrease.