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. 2021 Jul 10;12(1):167–181. doi: 10.1016/j.apsb.2021.07.002

Figure 3.

Figure 3

Astaxanthine protects against cisplatin-induced cell death via NRF2-mediated pathway. (A) Quantitative analysis of mRNA levels of Nrf 2, Hmox1, and Nqo1 in cochlear explants from different treatments. Data are presented as mean ± SD, ∗∗∗∗P < 0.0001 compared with control (blue) and CDDP (black) by one-way ANOVA with Bonferroni correction (n = 4–6 for each group; details shown in data excel). (B) Representative Western blot images of NRF2, HMOX1, and NQO1 in HEI-OC1 cell lines from different treatments. The protein band intensities relative to the loading control β-actin expression were measured by ImageJ software. Data are presented as mean ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 compared with control (blue) and CDDP (black) by one-way ANOVA with Bonferroni correction, n = 3 for each group. (C) Representative Myosin VIIA (green) and DAPI (blue) stained confocal images of postnatal 3-day C57BL/6 mouse cochlear explants from the control, ML385, AST/CDDP, and AST/CDDP plus ML385 (AST/CDDP/ML385) groups. Scale bars = 20 μm. (D) Quantitative analysis of mRNA levels of Nrf2 and Hmox1 in cochlear explants from different treatments. Data are presented as mean ± SD, ∗∗∗∗P < 0.0001 compared with the AST/CDDP/ML385 group by one-way ANOVA with Bonferroni correction (n = 3–4 for each group; details shown in data excel). ns, not significant; AST, astaxanthine; CDDP, cisplatin; Base, basal turn; Middle, middle turn; Apex, apical turn.