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. 2021 Jul 10;12(1):167–181. doi: 10.1016/j.apsb.2021.07.002

Figure 5.

Figure 5

Effects of astaxanthine on maintaining mitochondrial function in HEI-OC1 cell line and IHCs. (A) and (B) Representative MTRC (red) and DAPI (blue) stained confocal images of HEI-OC1 cells from the control, CDDP, AST, and AST/CDDP groups. The ImageJ was used for the analysis of quantitative changes in the mean MTRC fluorescence intensity. The data are presented as mean ± SD, ∗∗∗∗P < 0.0001 compared with control (blue) and CDDP (black) by one-way ANOVA with Bonferroni correction, n = 6 for each group in (B). Scale bar = 10 μm. (C) and (D) Representative MTRC (magenta) and DAPI (blue) stained confocal images of IHCs in middle regions of postnatal 3-day C57BL/6 mouse cochleae from different treatment groups. The Ortho section shows mitochondrial staining of IHCs and the ROI fluorescence from a single IHC in each group was identified under the same microscope settings (C). The ImageJ was used for the analysis of quantitative changes in the mean MTRC fluorescence intensity (D). The data are presented as mean ± SD, ∗∗∗P < 0.001 compared with control (blue) and CDDP (black) by one-way ANOVA with Bonferroni correction, n = 3 for each group in (D). Scale bar = 20 μm. ns, not significant; AST, astaxanthine; CDDP, cisplatin; IHC, inner hair cell; MTRC, MitoTracker Red CMXRos; ROI, region of interest.