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. 2021 Jul 8;12(1):406–423. doi: 10.1016/j.apsb.2021.07.001

Figure 3.

Figure 3

Internalization and intracellular distribution of NPs. (A) Penetration profiles and (B) the corresponding quantitative results (as mean fluorescence intensity, MFI) of N770-DOX@NPs in MCSs of A549 cells with or without NIR laser irradiation. Scale bars represent 100 μm. (C) Confocal images of cellular uptake profiles of N770-DOX@NPs in respective A549 cells, HUVECs, and 4T1 cells after incubation for 4 h. Nuclei were stained with DAPI (blue). Scale bar is 20 μm. (D) Percentages of DOX-containing A549 cells and (E) their corresponding MFIs after treatment with DOX@NPs, N770-DOX@NPs, and N770-DOX@NPs (+free N770) for 1, 2, and 4 h, respectively. (F) Percentages of DOX-containing 4T1 cells and (G) their corresponding MFIs after treatment with DOX@NPs, N770-DOX@NPs, and N770-DOX@NPs (+free N770) for 1, 2, and 4 h, respectively. (H) Percentages of DOX-contained HUVECs and (I) their corresponding MFIs after treatment with DOX@NPs, N770-DOX@NPs, and N770-DOX@NPs (+free N770) for 1, 2, and 4 h, respectively. These NP suspensions were prepared at equal DOX concentrations of 50 μg/mL. Data are expressed as mean ± SEM (n = 3; ∗P < 0.05; ∗P < 0.01; and ns = not significant). (J) Lysosomal escape properties of N770-DOX@NPs in A549 cells. Scale bar is 5 μm. (K) Mitochondrial co-localization profiles of N770-DOX@NPs in A549 cells. Scale bar is 20 μm.