Figure 6.
In vivo bio-distribution of NPs in mice bearing A549 lung carcinoma xenografts. (A) FLI of mice bearing A549 tumor after intravenous injection of free N770 solution and N770-DOX@NP suspension at an equal N770 concentration of 1 mg/kg (B) MFIs of tumor tissues from mice after treatment with free N770 and N770-DOX@NPs at different timepoints (6, 12, 24, 48, and 72 h). Data are expressed as mean ± SEM (n = 3; ∗P < 0.05; ∗∗P < 0.01; and ns = not significant). (C) Ex vivo FLI and (D) corresponding quantitative data of fluorescence intensities in the five major organs and tumor tissues from mice after the treatment with N770-DOX@NPs (N770: 1 mg/kg) at the timepoints of 24 and 72 h. Data are expressed as mean ± SEM (n = 3; ∗P < 0.05; ∗∗P < 0.01; and ns = not significant). (E) PAI of tumor tissues from mice after treatment with free N770 and N770-DOX@NPs at different timepoints (12, 24, 48, and 72 h). (F) PTI of mice bearing A549 tumor after intravenous injection of free N770 solution and N770-DOX@NP suspension at an equal N770 concentration of 1 mg/kg for 24 h upon 808 nm laser irradiation (0.8 W/cm2, 5 min), and (G) temperature profiles of tumors monitored by infrared thermal camera. Data are expressed as mean ± SEM (n = 3). (H) Variation of N770 concentrations in serum after intravenous injection of free N770 solution and N770-DOX@NP suspension at an equal N770 concentration of 1 mg/kg. Data are expressed as mean ± SEM (n = 3). (I) Quantification of drug amounts in the major organs and tumor tissues at different timepoints post injection (12, 24, 48, and 72 h). Data are expressed as mean ± SEM (n = 3).