REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CDK2 Rabbit monoclonal [E304]	Abcam	Cat# ab32147; RRID:AB_726775
Anti-Actin Antibody, clone C4	Millipore	Cat# MAB1501; RRID:AB_2223041
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5	Millipore	Cat# MAB374; RRID:AB_2107445
MAP LC3beta (G-2) antibody	Santa Cruz Biotechnology	Cat# sc-271625; RRID:AB_10714949
Lamin A/C antibody (polyclonal)	Proteintech	Cat# 10298-1-AP; RRID:AB_2296961
SQSTM1 (D-3) antibody	Santa Cruz Biotechnology	Cat# sc-28359; RRID:AB_628279
IRDye 680RD Donkey anti-Mouse IgG antibody	LI-COR Biosciences	Cat# 926-68072; RRID:AB_10953628
IRDye 800CW Donkey anti-Rabbit IgG antibody	LI-COR Biosciences	Cat# 926-32213; RRID:AB_621848
Progerin (13A4D4) antibody	Santa Cruz Biotechnology	Cat# sc-81611; RRID:AB_1128450
Lamin A/C (H-110) antibody	Santa Cruz Biotechnology	Cat# sc-20681; RRID:AB_648154
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11001; RRID:AB_2534069
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-11012; RRID:AB_2534079
ChIPAb + Histone H3 (C-term) antibody	Millipore	Cat# 17-10046; RRID:AB_10618160
ChIPAb + Trimethyl-Histone H3 (Lys36) antibody	Millipore	Cat# 17-10032; RRID:AB_10615601
ChIPAb + CTCF Validated Antibody and Primer Set	Millipore	Cat# 17-10044; RRID:AB_10732951
Chemicals, peptides, and recombinant proteins
Rapamycin	Sigma	553211
chloroquine	Abcam	ab139484
Bafilomycine A1	Sigma	5.08409
DAPI	Thermo Fisher Scientific	D1306
Ribonuclease A	Sigma	R5503
Lipofectamine™ RNAiMAX Transfection Reagent	Thermo Fisher Scientific	13778500
jetPRIME®	Polypus Transfection	114-15
Trypan blue 0.4%	Eurobio	CSTCOL03-0P
Critical commercial assays
Senescence β-galactosidase Staining kit	Cell Signaling Technology	9860
BrdU Cell Proliferation ELISA Kit	Abcam	ab126556
FxCycleTM Far Red Stain	Thermo Fisher Scientific	F10348
CellTox™ Green Cytotoxicity Assay	Promega	G8741
CellTiter-Glo® 2.0 Cell Viability Assay	Promega	G9241
Autophagy Assay Kit	Abcam	ab139484
Pierce™ BCA Protein Assay Kit	Thermo Fisher Scientific	23225
SYTOX™ AADvanced™ Dead Cell Stain Kit	Thermo Fisher Scientific	S10349
miRCURY LNA SYBR Green PCR Kit	Qiagen	339346
miRNeasy Mini Kit	Qiagen	217004
miRCURY LNA RT Kit	Qiagen	339340
Deposited data
miRNA expression profiling	This paper	https://doi.org/10.6084/m9.figshare.9642803.v1.
Experimental models: Cell lines
15-5968	CRB-TAC	N/A
13-13622	CRB-TAC	N/A
13-8243	CRB-TAC	N/A
13-13090	CRB-TAC	N/A
AG11513	Coriell	Cat# AG11513; RRID:CVCL_0Q97
GM01972	Coriell	Cat# AG01972; RRID:CVCL_F261
AG11498	Coriell	Cat# AG11498; RRID:CVCL_H766
GM08398	Coriell	Cat# GM08398; RRID:CVCL_7481
AG08498	Coriell	Cat# AG08498; RRID:CVCL_1Y51
AG07095	Coriell	Cat# AG07095; RRID:CVCL_0N66
AG08471	Coriell	Cat# AG08471; RRID:CVCL_1Y50
Experimental models: Organisms/strains
C57BL/6J LmnaG609G/G609GMus musculus	(Osorio et al., 2011)	N/A
C57BL/6J wild type Mus musculus	Charles river	N/A
Oligonucleotides
miRIDIAN microRNA Human hsa-miR-376a-3p - Mimic	Horizon discovery	C-300683-03-0010
miRIDIAN microRNA Human hsa-miR-376b-3p	Horizon discovery	C-300741-03-0010
miRIDIAN microRNA Mimic Negative Control	Horizon discovery	CN-001000-01-05
miR-376a-3p_MUT: Custom miRNA Mimic, miRIDIAN mimic	Horizon discovery	CTM-549257
miR-376b-3p_MUT: Custom miRNA Mimic, miRIDIAN mimic	Horizon discovery	CTM-549261
miRCURY LNA inhibitor control, negative control B	Qiagen	YI00199007
miRCURY LNA miRNA Power Inhibitor Control, Negative control A	Qiagen	YI00199006
miRCURY LNA miRNA Power Inhibitor hsa-miR-376a-3p	Qiagen	YI04100305
miRCURY LNA miRNA Power Inhibitor hsa-miR-376b-3p	Qiagen	YI04101339
Control scrambled Ex10-scrambled 50-ATCGGCTTGTCGCGTGAGCGATCGA-3	Gene Tools	MO-HSex10scrambled
Control scrambled Ex11-scrambled 50-ACCAGTGGCGTCGCCTCGCAGGTCC-30.	Gene Tools	MO-HsEx11SCRAMBLED
exon 10-lamin A splice donor site Ex10 (50-GCTACCACTCACGTGGTGGTGATGG-30)	Gene Tools	MO-HSEX10
Ex11 (50-GGGTCCACCCACCTGGGCTCCTGAG-30) bound the c.1824C > T; p.Gly609Gly-mutated sequence in the region of the LMNA transcript	Gene Tools	MO-HSEX11
SNORD110(mmu) miRCURY LNA miRNA PCR Assay	Qiagen	YP00203912
SNORD49A (hsa) miRCURY LNA miRNA PCR Assay	Qiagen	YP00203904
hsa-miR-376b-3p miRCURY LNA miRNA PCR Assay	Qiagen	YP00204218
hsa-miR-376a-3p miRCURY LNA miRNA PCR Assay	Qiagen	YP00204508
mmu-miR-376b-3p miRCURY LNA miRNA PCR Assay	Qiagen	YP00205058
mmu-miR-376a-3p miRCURY LNA miRNA PCR Assay	Qiagen	YP00205059
Primers for ddPCR see Table S2	This paper	N/A
RainbowFish Probes RP11-123M6 (green)	Amplitech	RP11-123M6 (green)
RainbowFish Probes RP11-123M6 (orange)	amplitech	RP11-123M6 (orange)
RainbowFish Probes RP11-815P21 (red)	amplitech	RP11-815P21 (red)
Recombinant DNA
ptfLC3 plasmid	(Kimura et al., 2007) Addgene	plasmid # 21074
pBABE puro EGFP plasmid	Oskar Laur, Addgene	plasmid # 128041
pBABE-puro-GFP-wt-lamin A plasmid	(Scaffidi and Misteli, 2006) Addgene	plasmid # 17662
pBABE-puro-GFP-progerin plasmid	(Scaffidi and Misteli, 2006) Addgene	plasmid # 17663
Software and algorithms
R	R	https://cran.r-project.org/mirrors.html
Image Lab Software	Bio-Rad	https://www.bio-rad.com/fr-fr/product/image-lab-software?ID=KRE6P5E8Z
ZEN 2	Carl Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
FlowJo software	FlowJo	https://www.flowjo.com/solutions/flowjo/downloads
Imaris software	Imaris	https://imaris.oxinst.com/products/imaris-for-cell-biologists
GraphPad Prism 8	GraphPad	https://www.scienceplus.com/nl/prism-commercieel.html
Exiqon GenEx® software	Exiqon	N/A