REAGENT	SOURCE	IDENTIFIER
Antibodies
Anti SNAP23	Synaptic System	111–213; RRID:AB_10805651
Anti SNAP23	Synaptic System	111–202; RRID:AB_887788
Anti KCC2 (D1R2R)	Cell Signaling Technology	94725; RRID:AB_2800232
Anti-KCC2 [N1/12]	Abcam	Cat# ab134300
Anti Syntaxin 1A	Synaptic Systems	110–111; RRID:AB_887848
Anti FLAG	Genscript	A 00187; RRID:AB_1720813
Anti Transferrin receptor	Thermofisher scientific	13–6800; RRID:AB_2533029
β-Actin	MP Biomedicals	Cat# 0869100
β-Tubulin	Santa Cruz Biotechnology	sc5274; RRID:AB_2288090
Chemicals, Peptides and recombinant proteins
Dulbecco's Modified Eagle's Medium (DMEM)	Biological Industries	01-055-1A
Fetal Calf Serum	Biological Industries	04-001-1A
pen-strep	Biological Industries	03-031-1B
L-Glutamine	Biological Industries	03-020-1B
Zinc sulfate heptahydrate	Sigma- Aldrich	z4750
BCECF-AM	Tef Labs	Not available
BSA	MP Biomedicals	216006980
TransIT-X2	mirusbio	MIR 6000
DIOA 2-[(2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-3H-inden-5-yl)oxy]acetic acid	Sigma-Aldrich	D129
Bumetanide 3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid	Sigma-Aldrich	B3023
Staurosporine	Santa Cruz Biotechnology	sc3510A
Go6983 3-[1-[3-(dimethylamino)propyl]-5-methoxyindol-3-yl]-4-(1H-indol-3-yl)pyrrole-2,5-dione	Sigma-Aldrich	G1918
BAY11-7085 (E)-3-(4-tert-butylphenyl)sulfonylprop-2-enenitrile	APExBio	B3033
VU0463271 N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-(6-phenylpyridazin-3-yl)sulfanylacetamide	Tocris	4719
Iodoacetamide 2-iodoacetamide	Sigma-Aldrich	I6125
Protease inhibitor cocktail	A2S	K1007
Papain 2-[[2-[[2-[(2-aminoacetyl)amino]acetyl]amino] -3-(4-hydroxyphenyl)propanoyl]amino]-5-(diaminomethylideneamino)pentanoic acid	Worthington	LS033126
Hanks' balanced salts solution	Biological Industries	02-015-1B
HEPES 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid	Biological Industries	03-025-1C
poly-D-lysine	Sigma-Aldrich	p0899
Neurobasal medium	GIBCO	21103049
Fetal Bovine Serum	HyClone	sh30070.03
B-27	GIBCO	17504044
Glutamax-100X	GIBCO	35050038
Gentamicin	Biological Industries	03-035-1C
Veriblot IP detection reagent	Abcam	ab131366; RRID:AB_2892718
protein A magbeads	GenScript	L00273
qPCR bio probe blue mix	PCR biosystem	pb20.25-05
Cytosine β-D-arabinofuranoside (AraC)	Sigma- Aldrich	c6645
SNAP23 blocking peptide	GRKKRRQRRRPQMDDLSPEEIQLRAHQVTDSEQ	GenScript USA Inc
SCR blocking peptide	GRKKRRQRRRPQGFAESLFQSIEKESGFSCG	GenScript USA Inc
Critical commercial assays
Bio-Rad protein assay	Bio-Rad	5000006
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	E0554S
EZ-Link Sulfo-NHS-Biotinylation Kit	Pierce Biotechnology, Thermoscientific	PIR-89881
Duolink in situ red starter kit	SigmaAldrich	DU092101
PureLink™ RNA Mini Kit	Invitrogene	12183018a
cDNA synthesis kit	Quanta bio	95047
Experimental models: Cell lines
HEK293	ATCC	CRL-1573
SHSY-5Y	ATCC	CRL-2266
Experimental models: Organisms/strains
C57BL/6JRccHsd mice	Envigo	043
Oligonucleotides
siGPR39	5′-CCAUGGAGUUCUACAGCAU-3′	Sigma- Aldrich
siSNAP23	5′-CCAACAGAGAUCGUAUUGA-3′	Integrated DNA Technologies, IDT
siKCC2	5′-CAAACAGAUGCAUUUAACCAAGAAT-3′	Integrated DNA Technologies, IDT
SCR	5'-GCCCAGAUCCCUGUACGU-3′	Sigma- Aldrich
KCC2 S940A	5′-GTCACGAGGCGCAATCCGGAG-3′	5′-TCATCTGTGATACTCTGGATCTC-3′
KCC2 S988A	5′-CAGCTCCCCGGCCCCAGGGGA-3′	5′-CTGGGGCAGCTGGGAGCACTC-3′
KCC2 S986A	5′-CCCCAGCAGCGCCCCGTCCCC-3′	5′-CAGCTGGGAGCACTCTGATCGTGG-3′
KCC2 T584A	5′-GCTGCTGAGGGCACCCAACTG-3′	5′-GTCTGCACTGCACAGGCC-3′
KCC2 T906E	5′-CTATGAGAAGGAGTTGGTGATGGAG-3′	5′-GTGTAAGCTGAGATGTCG-3′
KCC2 T1007E	5′-GGTGCATCTCGAGTGGACCAAGGACAAG-3′	5′-TTCTCCGGATCTGTCTCC-3′
KCC2 S868A	5′-GGATGACAATGCCATCCAGATGAAGAAG-3′	5′-ATCTGGGCCACAGTGAAG-3′
SPVSS	5′-GAGGGCATCAAGGACTTC-3′	5′-GGGGCCCTTATTCTTCTC-3′
ΔNCD-KCC2	5′-ATGGGCGTGTACCTGCCG-3′	5′-TTTATCATCATCATCTTTATAA TCCATAGCCGAATTC-3′
ΔCTD-KCC2	5′-TGCTCTAGAGCATCATATC CCATCGCCCCACTCC-3′
SNAP23 S95/120A	5′-GAACTTTGAGGCTGGAAAGAAC-3′	5′-TTTGTCCTATTACAAGGG-3′
5′-TAAGCAACCGGCCCGGATTACAAATG-3′	5′-GATACTACATTGCTAGGTG-3′
Recombinant DNA
human KCC2 cDNA	Prof. Gamba
mouse SNAP23	OriGene	MR202271
Human GPR39	OriGene- with mcherry addition and deletion of FLAG-MYC	www.origene.com/catalog/cdna-clones/expression-plasmids/rc219212/gpr39-nm_001508-human-tagged-orf-clone