REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human Fc specific antibody	Sigma-Aldrich	I1886; RRID:AB_260125
Polyclonal alkaline phosphatase-conjugated anti-human Fc antibody produced in goat	Sigma-Aldrich	A9544; RRID:AB_258459
monoclonal horseradish peroxidase-conjugated anti-GST antibody produced in goat	Rockland	600-103-200; RRID:AB_218192
Chemicals, peptides, and recombinant proteins
Human FcRn	In-house	NA
Recombinant human IL-12	PeproTech	200-12
Mouse epidermal growth factor (BD	ThermoFisher	PMG8043
MES	Sigma-Aldrich	M3671
NaCl	Sigma-Aldrich	S9888
Tris/HCl	Sigma-Aldrich	T3253
Na2HPO4x2H2O,	Sigma-Aldrich	71645
NaH2PO4xH2O	Sigma-Aldrich	S9638
3,3′5,5′-tetramethylbenzine substrate	Merck	CL07
Tween-20	Sigma-Aldrich	P1379
Skimmed milk	ITW reagents	A0830
phosphate-buffered saline	Sigma-Aldrich	D8537
Hydrocortisone	Sigma-Aldrich	H0888
G418	ThermoFisher	10131027
Blasticidin S	ThermoFisher	A1113903
Alkaline phosphatase substrate	Sigma-Aldrich	S0942
K3-EDTA	Sigma-Aldrich	03664
cOmplete protease inhibitor cocktail	Roche	11836145001
NT-647-HNS fluorescent dye	Nanotemper technologies	MO-L001
Phosphate-buffered saline	Sigma-Aldrich	D8537
MCDB 131 medium	Gibco	10372019
RPMI medium	Sigma-Aldrich	R2405
Heat-inactivated fetal calf serum	Sigma-Aldrich	F7524
L-glutamine	ThermoFisher	25030123
Streptomycin and penicillin	Sigma-Aldrich	P4458
Hank’s Balanced salt solution	ThermoFisher	14025100
MEM non-essential amino acids	ThermoFisher	11140050
RIPA buffer	ThermoFisher	89900
Hank’s Balanced salt solution	ThermoFisher	14025100
Experimental models: Cell lines
HEK293E	ATCC	CRL-1573
HMEC1 stably expressing HA-hFcRn-EGFP (HMEC1-hFcRn	Weflen et al., 2013	Harvard Medical School and Harvard Digestive Diseases Center
Experimental models: Organisms/strains
Hemizygous hFcRn transgenic Tg32 mice, B6.Cg-Fcgrttm1DcrTg(FCGRT)32Dcr/DcrJ	The Jackson Laboratory	NA
FcRn-deficient B6-Fcgrttm1Dcr mice	The Jackson Laboratory	NA
Oligonucleotides
Control siRNA	Santa Cruz Biotechnology Inc.	sc-37007
Mixture of three hFcRn HC-specific siRNAs	Santa Cruz Biotechnology Inc	sc-45632
Other
ustekinumab Fab	RCSB PDB (www.rcsb.org)	3HMW
briakinumab Fab	RCSB PDB	5N2K
ustekinuamb-IL-12 complex	RCSB PDB	3HMX
briakinumab-IL23 complex	RCSB PDB	5NJD
Software and algorithms
GraphPad Prism 7	GraphPad Software Inc	https://www.graphpad.com/scientific-software/prism/
Jalview 2	Waterhouse et al., 2009	Jalview.org
PyMOL	PyMOL by Schrödinger	Pymol.org
Emboss iep	Emboss	http://www.bioinformatics.nl/cgi-bin/emboss/iep?_pref_hide_optional=0
pdb2pqr sever	Dolinsky et al., 2004	http://nbcr-222.ucsd.edu/pdb2pqr_2.1.1/
Other
24 well plates	Costar	10732552
96-well ELISA plates	Costar	10544522
CaptureSelecet™ pre-packed anti-human IgG-CH1 column	Life technologies	494346201
Amicon Ultra-15 mL 50K columns	Millipore	C7715
Superdex 200 increase 10/300 GL column	GE Healthcare	28–9909-44
Amicon Ultra-0.5 mL 100K columns	Millipore	UFC510096
SDS-PAGE	ThermoFisher	NW00125BOX
MycoAlertTM PLUS Mycoplasma detection kit	Lonza	LT07-703
analytical FcRn column	Roche	08128057001
GSTrap HP column	GE Healthcare	17–5282-02
HisTrap HP column supplied with Ni2+ ions	GE Healthcare	17–5027-68