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. 2021 Dec 30;298(2):101552. doi: 10.1016/j.jbc.2021.101552

Figure 2.

Figure 2

Alpha-synuclein and phospho-synuclein bind DNA with blunt or 5′ overhanging ends with similar affinity. Alpha-synuclein (aSyn, A) and serine-129 phosphorylated alpha-synuclein (pSyn, B) bind 300 bp DNA with blunt or 300 bp DNA with a 4 base 5′ overhanging end on both sides similarly (shifted fraction blunt end at 0–4, 16, 29, and 57 μM aSyn: 0.000 ± 0.000, 0.007 ± 0.012, 0.387 ± 0.110, 0.857 ± 0.006; shifted fraction overhanging ends at 0–14, 29 and 57 μM aSyn: 0.000 ± 0.000, 0.417 ± 0.121, 0.917 ± 0.031; comparison of shifted fraction of blunt and overhanging end DNA for each aSyn concentration by paired t test shows no significant differences, p between 0.059 and 0.423; variable slope dose-normalized response curve; N = 3 gels, x-axis aSyn concentration on log scale; shifted fraction blunt end at 0–14, 29 and 57 μM pSyn: 0.000 ± 0.000, 0.250 ± 0.098, 0.607 ± 0.101; shifted fraction overhanging ends at 0–14, 29 and 57 μM pSyn: 0.000 ± 0.000, 0.217 ± 0.188, 0.573 ± 0.161; comparison shifted fraction of blunt and overhanging end DNA for each pSyn concentration by paired t test shows no significant differences, p between 0.469 and 0.604; variable slope dose-normalized response curve; N = 3 gels, x-axis pSyn concentration on log scale).