REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
| ||
D-glucose | VWR | Cat. #0188 |
Acetate | Sigma | Cat. #S8625 |
D-lactate | Sigma | Cat. #71716 |
succinate | Alfa Aesar | Cat. #419A3 |
| ||
Critical commercial assays | ||
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glucose GO assay kit | Millipore | Cat. #GAGO20 |
Acetate assay kit | Abcam | Cat. #ab204719 |
D-Lactate assay kit | Abcam | Cat. #ab83429 |
Succinate assay kit | Abcam | Cat. #ab204718 |
DNeasy 96 Blood & Tissue kit | QIAGEN | Cat. #69582 |
Quan-iTPicoGreen dsDNA Assay kit | Invitrogen | Cat. #P11496 |
SequalPrep PCR cleanup and normalization kit | Invitrogen | Cat. #A1051001 |
| ||
Deposited data | ||
| ||
16S rRNA sequencing abundance data | This study | https://doi.org/10.5061/dryad.5x69p8d3z |
Genome scale metabolic models | This study | SRA: PRJNA749600 |
Raw 16S rRNA amplicon sequences for communities assembled in glucose under different migration treatments | This study | SRA: PRJNA761777 |
Raw 16S rRNA amplicon sequences for communities assembled in alternate carbon sources | This study | SRA: PRJNA761387 |
Community abundance data | Goldford et al., 2018 | https://doi.org/10.5281/zenodo.3817698 |
| ||
Software and algorithms | ||
| ||
Source data files and code used to generate the figures. | This study |
https://github.com/sylestrela/Estrelaetal2021_FunctionalAttractors (https://doi.org/10.5281/zenodo.5510318) |
Code for the FBA simulations. | This study |
https://github.com/vilacelestin/Estrelaetal2021_FunctionalAttractors (https://doi.org/10.5281/zenodo.5510298) |
R (version 3.4.3) | (R Core Team, 2017) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.r-project.org/ |
RRID:SCR_001905 |
DADA2 (version 1.6.0) | Callahan et al., 2016 | N/A |
COBRApy (version 0.17.1) | Ebrahim A, Lerman JA, Palsson BO, Hyduke DR. COBRApy: COnstraints-Based Reconstruction and Analysis for Python. BMC Syst Biol. 2013;7: 74. |
RRID:SCR_012096 |
Mathematica (version 11.0.1.0) | Wolfram | RRID:SCR_014448 |