KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| D-glucose | VWR | Cat. #0188 |
| Acetate | Sigma | Cat. #S8625 |
| D-lactate | Sigma | Cat. #71716 |
| succinate | Alfa Aesar | Cat. #419A3 |
|
| ||
| Critical commercial assays | ||
|
| ||
| glucose GO assay kit | Millipore | Cat. #GAGO20 |
| Acetate assay kit | Abcam | Cat. #ab204719 |
| D-Lactate assay kit | Abcam | Cat. #ab83429 |
| Succinate assay kit | Abcam | Cat. #ab204718 |
| DNeasy 96 Blood & Tissue kit | QIAGEN | Cat. #69582 |
| Quan-iTPicoGreen dsDNA Assay kit | Invitrogen | Cat. #P11496 |
| SequalPrep PCR cleanup and normalization kit | Invitrogen | Cat. #A1051001 |
|
| ||
| Deposited data | ||
|
| ||
| 16S rRNA sequencing abundance data | This study | https://doi.org/10.5061/dryad.5x69p8d3z |
| Genome scale metabolic models | This study | SRA: PRJNA749600 |
| Raw 16S rRNA amplicon sequences for communities assembled in glucose under different migration treatments | This study | SRA: PRJNA761777 |
| Raw 16S rRNA amplicon sequences for communities assembled in alternate carbon sources | This study | SRA: PRJNA761387 |
| Community abundance data | Goldford et al., 2018 | https://doi.org/10.5281/zenodo.3817698 |
|
| ||
| Software and algorithms | ||
|
| ||
| Source data files and code used to generate the figures. | This study |
https://github.com/sylestrela/Estrelaetal2021_FunctionalAttractors (https://doi.org/10.5281/zenodo.5510318) |
| Code for the FBA simulations. | This study |
https://github.com/vilacelestin/Estrelaetal2021_FunctionalAttractors (https://doi.org/10.5281/zenodo.5510298) |
| R (version 3.4.3) | (R Core Team, 2017) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.r-project.org/ |
RRID:SCR_001905 |
| DADA2 (version 1.6.0) | Callahan et al., 2016 | N/A |
| COBRApy (version 0.17.1) | Ebrahim A, Lerman JA, Palsson BO, Hyduke DR. COBRApy: COnstraints-Based Reconstruction and Analysis for Python. BMC Syst Biol. 2013;7: 74. |
RRID:SCR_012096 |
| Mathematica (version 11.0.1.0) | Wolfram | RRID:SCR_014448 |