Fig. 4.

NAC and GSH impaired variant RBD binding to ACE2 in vitro.

(A) Dose-dependent inhibition of Wuhan-type recombinant RBD proteins binding to ACE2 by NAC and GSH.(B) ACE2-binding activity of variants RBD protein was inhibited by NAC. (C) ACE2-binding activity of variants RBD protein was inhibited by GSH.Above experiments were repeated independently twice and similar results were observed. Data from triplicated samples were expressed as the means ± SD. Student's t-test was performed to assess statistical significance. ∗∗ indicates p < 0.01.