Fig.3.

5-HT_2BR is required for the effects of fluoxetine on activation of A1 astrocyte in vitro. A Expression of 5-HT_2BR on primary cultured microglia, astrocyte and neuron. B Expression of 5-HT_2BR on astrocyte in the hippocampus. Similar results were obtained in 3 separate experiments. C, D Effects of 5-HT_2BR inhibitor, RS127445, on the expression of A1-special transcripts in astrocytes with or without fluoxetine treatment after A1 cocktail C or IL-6 D stimulation for 24 h. E Effects of 5-HT_2BR knockdown on the mRNA levels of A1-special markers in astrocytes with or without fluoxetine treatment after IL-6 stimulation for 24 h. Scale bars, 20 µm in B. Quantitative data are mean ± s.e. Data were analyzed using two-way ANOVA, then combined with Tukey’s multiple comparison to assess the differences between groups. All data represent the results of three independent experiments. C–E: **P < 0.01, and ***P < 0.001 vs respective Control cells treated with PBS; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs Control cells treated with A1 (C) or IL-6 (D, E) stimulation; ^$P < 0.05, ^$$P < 0.01, and ^$$$P < 0.001