Fig. 4.

Eriodictyol inhibits ferroptosis in the brains of APP/PS1 mice. A Iron accumulation in the brains of mice was stained with Prussian blue. B The levels of ferrous iron and total iron in the mouse cortex and hippocampus were detected using an iron assay kit. C The expression of TfRC, FTH and Fpn in the mouse cortex and hippocampus was measured using Western blotting, and β-actin served as a loading control. D MDA contents in the mouse cortex and hippocampus were detected using a Lipid Peroxidation MDA Assay Kit. E The GPX4 expression level in the mouse cortex and hippocampus was measured using Western blotting. The data are presented as the means ± SD of three experiments. **P < 0.01 and ***P < 0.001 compared with the control group