Table 1.
Primers for cDNA construction of NDV containing PTEN CDS
| Gene | Direction | Sequence (5′ → 3′) | Size (bp) |
|---|---|---|---|
|
Kozak PTEN for Position “1” (1st PCR) |
Forward | GCCACCATGACAGCCATCATCAAAG | 1221 |
| Reverse | AGAAGCGGCCGCGGCCGGCCACCATGACAGCC | ||
|
Fse I Kozak PTEN for Position “1” (2nd PCR) |
Forward | GCCTCAGACTTTTGTAATTTGTGTATG | 1258 |
| Reverse | GTTGGACCTTGGTATGGCCGGCCTCAGACTTTTGTAATTTGTG | ||
|
Kozak PTEN for Position “2” (1st PCR) |
Forward | GCCACCATGACAGCCATCATCAAAG | 1221 |
| Reverse | CGCTCAGACTTTTGTAATTTGTGTA | ||
|
Not I Kozak PTEN for Position “2” (2nd PCR) |
Forward | ATACGGGTAGAAGCGGCCGCCACCATGACAGCC | 1254 |
| Reverse | TGGACCTTGGGCGGCCGCTCAGACTTTTGTAATTTG |