Table 3.
Primers for real-time quantitative PCR
| Gene | Direction | Sequence (5′ → 3′) | Size(bp) |
|---|---|---|---|
|
PTEN Real-Time |
Forward | TCCCAGTCAGAGGCGCTATGT | 152 |
| Reverse | GGCAGACCACAAACTGAGGA | ||
| Probe | [FAM]TGCAAGTTCCGCCACTGAACA[HEX] | – | |
|
P-Akt Real-Time |
Forward | ACGGGCACATTAAGATCACAGACT | 240 |
| Reverse | CCTCCATGAGGATGAGCTCAAAAAGC | ||
| Probe | CAATGACTACGGCCGTGCAGT[HEX] | – | |
|
hTERT Real-Time |
Forward | CAGACGGTGTGCACCAACATCT | 134 |
| Reverse | GTGTCAGAGATGACGCGCAGG | ||
| Probe | CAGCAAGTTTGGAAGAACCCCACA[HEX] | – | |
|
IFNA Real-Time |
Forward | CAGTTCCAGAAGGCTCAAGCCA | 304 |
| Reverse | TTTCTGCTCTGACAACCTCCCA | ||
| Probe | GAGAAGAAATACAGCCCTTGTGCC[HEX] | – | |
|
B-actin Real-Time |
Forward | TGAAGTGTGACGTGGACATC | 151 |
| Reverse | GGAGGAGCAATGATCTTGAT | ||
| Probe | ACGCCAACACAGTGCTGTCTG[HEX] | – |