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Everolimus inhibited invasion of 3D-spheroids formed by DCIS cells, MCF-10DCIS.COM or SUM225, through Matrigel. A. Representative Images were acquired with a 20× objective showing invasive extensions from the 3D-spheroids in the control group, but not in the everolimus treatment groups. B. The invasive percentage was calculated by No. of invasive 3D-organoid / No. of total 3D-organoid X 100. Data are shown as means ± SEM, n=3. **p<0.01 vs Vehicle control group with one-way ANOVA.
