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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Mol Immunol. 2021 Dec 23;142:1–10. doi: 10.1016/j.molimm.2021.12.011

Figure 1: mRNA Microarray analysis of Mo-MDSC.

Figure 1:

A) Flow cytometry shows the gating strategy for sorting cell populations that were isolated and used for mRNA generation and subsequent microarray analysis. B) qPCR for selected targets identified in microarray analysis (based upon likelihood of surface, intracellular and nuclear expression, as well as availability of antibodies) was performed to validate mRNA enrichment in Mo-MDSC versus CD14+ HLA-DR+.