Figure 6. The suppressive capacity of CD14⁺ VNN2^high cells on activated CD8 T cells was comparable to traditional (CD14⁺ HLA-DR^neg/low) MDSC.

A) Representative functional suppression assay showing selected cell populations from CD14⁺ magnetically selected PBMCs. B) Panel I) and II) show suppression of proliferating CD2/CD3/CD28-stimulated, e670 labeled CD8 T cells by CD14⁺ VNN2^high cells (panel I) is nearly equivalent to Mo-MDSC (panel II). Panels III) and IV) show the suppressive capacity of either CD14⁺ VNN2^low or CD14⁺ HLA-DR^high in mixed culture with stimulated CD8 T cells, respectively. C) Cumulative data for suppression assays based upon normalization to maximally stimulated CD8 T cell proliferation (n=8). D) Representative histogram demonstrating the selection of VNN2-expressing CD14⁺ cells. E) Percent suppression of selected subsets of VNN2 expressing cells used in a functional suppression assay with anti-CD2/CD3/CD28 bead stimulation of CD8 T cells at a 1:2 ratio of VNN2⁺ cells to CD8 T cells (n=2 represent two independent experiments from different batches of cells).