FIGURE 3.

Acetylation of K416 increases the interaction of Abi1 with N‐WASP and N‐WASP phosphorylation in smooth muscle cells. A, Schematic diagram of Abi1 structural domains. Abi1 consists of several domains including WAB (WAVE‐binding domain), SNARE (SNARE‐binding domain), HHR (homeodomain homologous region), PRO (proline‐rich domain), and SH3 (Src homology 3). There are five lysines in the SH3 domain. PxxP, proline. B, Abi1 KD cells were transfected with wild‐type (WT) or various Abi1 mutants, and cell extracts were immunoprecipitated with Abi1 antibody and blotted with antibodies against Abi1 and N‐WASP. Data are mean values of three batches of cell culture. Error bars indicate SE. C, Extracts of cells expressing wild‐type (WT) or various mutants were blotted with antibodies against pN‐WASP (Y256) and total N‐WASP. Data are mean values of three batches of cell culture. Error bars indicate SE. *P < .01