Table 6.
Insertions that developed in the course of the experiment.
| Strain | aa position | nc position | Sequence | Length | Type of insertion | Location |
|---|---|---|---|---|---|---|
| A607∗ | S:22116-AAA...ACT-22117 | 22216 | AAAAAGTCTACT | 12 | Novel non-codon-aligned insertion | S1/NTD |
| A607 | S:214-RQSR-215 | 22204 | CGGCAGTCGAGA | 12 | Novel codon-aligned insertion | S1/NTD |
| A6301 | S:22199-TAC…GTT-22200 | 22199 | ACAGAAGAGGTT | 12 | Novel non-codon-aligned insertion | S1/NTD |
| A4707 | S:214-RQSR-215 | 22204 | CGGCAGTCGAGA | 12 | Novel codon-aligned insertion | S1/NTD |
| A818 | S:22206-GAG…AAT-22207 | 22206 | GAGAAGAAT | 9 | Novel non-codon-aligned insertion | S1/NTD |
Four of ten endemic strains of SARS-CoV-2 developed 9 to 12mer nucleotide insertions. One strain (A607) developed two different insertions sequentially. The nucleotide sequences, positions and translations into amino acid codons are shown here.
The insertion labelled with a “∗” is a temporary insertion, later replaced by S:214-RQSR-215.
nc, nucleotide; length, nucleotide length; aa, amino acid; NTD, N-terminal domaine; S1, spike 1.