KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| S. Typhimurium, SL1344 StrepR | Hoiseth and Stocker, 1981 | SL1344 |
| S. Typhimurium, SL1344 ΔprpC | This study | WJ207 |
| S. Typhimurium, SL1344 ΔprpC phoN::KanR | This study | WJ216 |
| S. Typhimurium, SL1344 ΔprpBCDE | This study | WJ195 |
| S. Typhimurium, SL1344 ΔprpBCDE phoN::KanR | This study | WJ177 |
| S. Typhimurium, SL1344 ΔinvA | This study | WJ227 |
| S. Typhimurium, SL1344 ΔinvAΔspiB | This study | WJ231 |
| S. Typhimurium, SL1344 ΔinvA ΔspiB ΔprpC | This study | CS019 |
| S. Typhimurium, SL1344 ΔinvA ΔspiB ΔprpC phoN::KanR | This study | CS031 |
| S. Typhimurium, SL1344 ΔnapA ΔnarZ narG::pCAL | Lopez et al., 2012 | CAL55 |
| S. Typhimurium, SL1344 ΔnapA ΔnarZ narG::pCAL5 phoN:: CmR | This study | CS048 |
|
S. Typhimurium, SL1344 ΔnapA ΔnarZ narG::pCAL5 ΔprpC |
This study | CS045 |
|
S. Typhimurium, SL1344 ΔnapA ΔnarZ narG::pCAL5 ΔprpC phoN:: KanR |
This study | CS046 |
| S. Typhimurium, SL1344 ΔcyxA | This study | WJ378 |
| S. Typhimurium, SL1344 ΔcyxA ΔprpC | This study | WJ380 |
| S. Typhimurium, SL1344 ΔsopE | This study | WJ377 |
| S. Typhimurium, SL1344 ΔsopE ΔprpC | This study | WJ379 |
| S. Typhimurium ATCC14028 NalR | Stojiljkovic et al., 1995 | IR715 |
| S. Typhimurium, IR715 ΔprpC | This study | NS017 |
| S. Typhimurium, IR715 ΔprpC::KanR | This study | NS039 |
| E. coli, S17-1 λpir, zxx::RP4 2-(TetR::Mu) (KanR::Tn7) λpir recA1 thi pro hsdR (r−m+) | (Simon et al., 1983) | S17-1 λpir |
| B. thetaiotaomicron, VPI 5482 Δtdk | Koropatkin et al., 2008 | VPI5482 |
| B. thetaiotaomicron, Δtdk Δ1686-1689 | Kovatcheva-Datchary et al., 2015 | BT1686-89 |
| Chemicals, peptides, and recombinant proteins | ||
| LB Broth, Miller (Luria Bertani) | Becton Dickinson | Cat# 244520 |
| LB Agar, Miller (Luria Berani | Becton Dickinson | Cat# 244620 |
| Platinum PCR Supermix High Fidelity | Invitrogen | 12532016 |
| PCR Supermix | Invitrogen | 10572014 |
| Bacto Brain Heart Infusion | Becton Dickinson | Cat# 237500 |
| Sheep Blood | Fischer BioReagents | Cat# R54008 |
| Sodium Nitrate | Sigma-Aldrich | Cat# S5506 |
| Potassium tetrathionate | Sigma-Aldrich | Cat# P2926 |
| Trimethylamine N-oxide | Sigma-Aldrich | Cat# T0514 |
| Sodium Fumarate | Alfa Aesar | Cat# 1127622 |
| Sodium Propionate | Alfa Aesar | Cat# 1744036 |
| Glycerol | Fisher BioReagents | Cat# G33-4 |
| D (+)- Glucose | Acros Organics | Cat# 410950010 |
| Isopropyl β-D-1-thiogalactopyranoside | Fischer BioReagents | Cat# BP1755 |
| iQ SYBR Green Supermix | Bio-Rad | 170-880 |
| Mucin from porcine stomach, Type II | Sigma-Aldrich | Cat# M2378 |
| Aminoguanidine HCl | Sigma-Aldrich | Cat# 396494 |
| Vanadium (III) Chloride | Acros Organics | Cat# 1970000250 |
| Sulfanilamide | Fischer BioReagents | Cat# 04525-100 |
| N- (1-naphthyl)ethylenediamine dichloride | Sigma-Aldrich | Cat# 222488 |
| Tri Reagent | Molecular Research | Cat# TR118 |
| Critical commercial assays | ||
| Gibson Assembly | NEB | Cat# E2611L |
| SurePrep TrueTotal R.N.A. Purification Kit | Fisher BioReagents | Cat# BP2800 |
| iScript gDNA Clear cDNA synthesis kit | Bio-Rad | Cat# 1725035 |
| Zymoclean Gel DNA Recovery Kit | Zymo Research | D4001 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6 | Jackson Laboratory | Cat# 000664 |
| Mouse: CBA | Jackson Laboratory | Cat# 000656 |
| Germ-free Swiss Webster | Byndloss Lab | N/A |
| Oligonucleotides | ||
| Primers used in this study, see Table S1 | This study | N/A |
| Recombinant DNA | ||
| Plasmid: pRDH10, ori(R6K) mobRP4 sacRB CmR TetR | Kingsley et al., 1999 | pRDH10 |
| Plasmid: pKD46, CarbR PBAD-gam-beta-exo oriR101 repA101ts | Datsenko and Wanner, 2000 | N/A |
| Plasmid: pKD13, KanR FRT KmR FRT PS1 PS4 oriR6Kγ | Datsenko and Wanner, 2000 | N/A |
| Plasmid: pCP20, CarbR CmR c/857 λPRflp oripSC101ts | Datsenko and Wanner, 2000 | N/A |
| Plasmid: Up- and downstream region of prpC (SL1344) in pRDH10 | This study | pWJ65 |
| Plasmid: Up- and downstream region of prpBCDE (SL1344) in pRDH10 | This study | pWJ50 |
| Software and algorithms | ||
| GraphPad Prism 8 | GrapPad Software | N/A |