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Knockdown of HE4 inhibited myocardial fibroblast fibrosis. (a) Identification of primary rat myocardial fibroblast (×400, 25 μm). (b) Expression of HE4 was tested by qRT-PCR and Western blot. (c) Acta2 expression was tested by qRT-PCR. (d) IF determined α-SMA expression (×400, 25 μm). (e) qRT-PCR and Western blot were utilized to detect collagen I, collagen III, MMP2, and MMP9 levels. ∗P < 0.05 vs. control; #P < 0.05 vs. si-NC.
