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. 2021 Dec 3;163(1):45–56. doi: 10.1530/REP-21-0332

Figure 7.

Figure 7

Label-free CARS 3D images in living MII oocytes matured with high PA. For IVM, immature GV stage oocytes were incubated overnight in standard M2 medium supplemented with 200 µM PA. MII oocytes were then selected for CARS imaging. (A) Single z step CARS image (xy section) of the oocyte at wavenumber 2850 cm−1. (B) Depth colour-coded images of CARS z-stacks through the same oocytes. (C) The CARS z-stacks of the same oocyte are presented as xz section, CARS was acquired; 0.1 × 0.1 μm xy pixel size; 0.5 μm z-step; 0.01 ms pixel dwell time; ~14 mW (~9 mW) pump (Stokes) power at the sample. Scale bars: 10 μm. Colour bar shows depth colour-coding from −25 to 25 μm of 101 z-stacks (0 μm being the approximately equatorial plane of the oocyte or embryo); the brightness of each colour is the maximum intensity at each corresponding z-plane. Scale bars: 5 μm.