Figure 4.

Establishment of an endometrial assembloid model. Schematic representation of the establishment of the endometrial assembloid model. Endometrial pipelle biopsies are digested and separated into epithelial and stromal fractions. Epithelial cells are expanded in Matrigel using the simple gland organoid culture approach for two passages, while stromal cells are expanded in monolayer culture. At passage 2, digested organoids and stromal cells are combined and encapsulated in collagen hydrogel to form an assembloid culture which then follows growth and differentiation protocols as required. Current and future applications for the assembloids include, but are not limited to embryo implantation studies, pharmaceutical testing (e.g. drugs or small molecules) and genetic modification of the different cell populations.