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. 2022 Jan 29;20:56. doi: 10.1186/s12951-022-01245-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Exo-Gel promoted proliferation and migration of mBMSCs in vitro. A In vitro setup to determine if Exo released from Exo-Gel maintain their ability in proliferation and migration of mBMSCs. B The cell viability of mBMSCs cocultured with conditioned medium examined by CCK-8 analysis. Data are expressed as means ± SEM (n = 6). *p < 0.05, Exo-Gel group compared to Gel group. C Proliferation of mBMSCs was confirmed by EDU kit assay (bar = 100 μm). D Quantification of rate of EDU positive cells in C was performed. Data are expressed as means ± SEM (n = 8). (E) Transwell assay to show a representative image of migrating mBMSCs (bar = 200 μm). F Quantitative analysis of migrating cells in E. Data are expressed as means ± SEM (n = 6). G A representative image showing the migration ability of mBMSCs by the scratch test (bar = 500 μm). (H) Quantitative analysis of mBMSC migration rate (G). Data are expressed as means ± SEM (n = 6). *p < 0.05