Table 1:
Example worklist for Targeted Analysis and quantitation of PFAS using LC-MS/MS
| ID | Sample Name | Sample Type | Std Conc | Vial | LC Method | MS Method |
|---|---|---|---|---|---|---|
| 1 | DB_001 | Blank | 1:A,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 2 | DB_002 | Blank | 1:A,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 3 | DB_003 | Blank | 1:A,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 4 | DB_004 | Blank | 1:A,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 5 | DB_005 | Blank | 1:A,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 6 | FB | Blank | 1:A,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 7 | 10 std | Standard | 10 | 1:A,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 8 | 25 std | Standard | 25 | 1:A,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 9 | 50 std | Standard | 50 | 1:A,5 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 10 | 100 std | Standard | 100 | 1:A,6 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 11 | 250 std | Standard | 250 | 1:A,7 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 12 | 500 std | Standard | 500 | 1:A,8 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 13 | 750 std | Standard | 750 | 1:B,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 14 | 1000 std | Standard | 1000 | 1:B,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 15 | DB_006 | Blank | 1:B,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 16 | SB_DUP1 | Analyte | 1:B,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 17 | SB_DUP2 | Analyte | 1:B,5 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 18 | SW Site 03 | Analyte | 1:B,6 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 19 | SW Site 16 | Analyte | 1:B,7 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 20 | SW Site 30 | Analyte | 1:B,8 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 21 | DB_007 | Analyte | 1:C,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 22 | SW Site 19 | Analyte | 1:C,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 23 | SW Site 48 | Analyte | 1:C,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 24 | SW Site 49 | Analyte | 1:C,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 25 | SW Site 05 | Analyte | 1:C,5 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 26 | SW Site 47 | Blank | 1:C,6 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 27 | DB_008 | Analyte | 1:C,7 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 28 | SW Site 19_DUP | Analyte | 1:C,8 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 29 | SW Site 20 | Analyte | 1:D,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 30 | SW Site 21 | Analyte | 1:D,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 31 | SW Site 46 | Analyte | 1:D,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 32 | SW Site 47 | Analyte | 1:D,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 33 | DB_009 | Blank | 1:D,5 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 28 | SW Site 32 | Analyte | 1:D,6 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 29 | SW Site 50 | Analyte | 1:D,7 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 30 | SW Site 25 | Analyte | 1:D,8 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 31 | SW Site 21_DUP | Analyte | 1:E,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 32 | SW Site 52 | Analyte | 1:E,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 33 | DB_010 | Blank | 1:E,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 34 | FB | Blank | 1:A,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 35 | 10 std | Standard | 10 | 1:A,3 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 36 | 25 std | Standard | 25 | 1:A,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 37 | 50 std | Standard | 50 | 1:A,5 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 38 | 100 std | Standard | 100 | 1:A,6 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 39 | 250 std | Standard | 250 | 1:A,7 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 40 | 500 std | Standard | 500 | 1:A,8 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 41 | 750 std | Standard | 750 | 1:B,1 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 42 | 1000 std | Standard | 1000 | 1:B,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |
| 43 | DB_011 | Blank | 1:B,2 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min | |
| 44 | DB_012 | Blank | 1:E,4 | PFAS grad 400uL/min - 9 min run | PFCMXA + HFPO-DA MS/MS - 9 min |