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. Author manuscript; available in PMC: 2022 Jan 30.
Published in final edited form as: J Vis Exp. 2019 Apr 18;(146):10.3791/59142. doi: 10.3791/59142

Table 2:

Example gradient for LC separation in targeted analysis

Time
(min)
0		 % A
(2.5mM Ammonium Acetate in 5% MeOH)
90		 % B
(2.5mM Ammonium Acetate in 95% MeOH)
10
5 15 85
5.1 0 100
7 0 100
7.1 90 10
9 90 10