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. Author manuscript; available in PMC: 2022 Jan 30.
Published in final edited form as: Curr Protoc Cytom. 2020 Jun;93(1):e74. doi: 10.1002/cpcy.74

Table 1.

Single-cell suspension from Spleen and Lung

Sample ID Sample volume (μL) Bead concentration/μL # Beads # Live Leukocytes Acquired Volume of Beads Total # Live Leukocytes Desired # Cells/mL Volume to Add (mL)
Spleen 800 1040 590 28,933 0.57 4.08E7 2E7 1.24
Lung 400 1040 2,519 21,942 2.42 3.62E6 5E6 0.32