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. 2022 Jan 1;27:810–823. doi: 10.1016/j.omtn.2021.12.033

Figure 6.

Figure 6

Efficient CRISPR-mediated transition of EGFP to EBFP by a.Gag.MS2.CRISPR.HDR particles

(A) Determined copies of CRISPR RNA and PT-modified ssODN molecules within a.Gag.MS2.CRISPR.HDR supernatants. (B and C) HT1080-based (B) or NuFF-based (C) EGFP reporter cells were transduced with 30 μL (1,980 copies SpCas9.TS mRNA/cell) of a.Gag.MS2.CRISPR.HDR supernatants in the absence (w/o) or presence of NHEJ or HDR inhibitors (inh) M3814 or YU238259, respectively. DMSO-treated cells served as solvent controls. The graphs display % EBFP+ cells within treated cultures. Non-treated or DMSO-treated mock cultures indicate the degree of autofluorescence in the EBFP channel. Each data point reflects one independently prepared supernatant (n = 3–4).