Figure 5.

Dissociation of the AMH complex correlates with bivalent binding to AMHR2.A, a mechanism for the receptor-induced dimerization of AMHR2 by c-AMH; monomeric and bivalent interactions are labeled RL or RLR, respectively. The relationships between mAb-N₁ or mAb-C₁ and RL and RLR are shown below the diagram. B, schematic diagrams showing the two experimental conditions for testing whether bivalent binding of AMH by AMHR2 induces prodomain displacement. On the left, the mAbs and secondary Ab are added individually, and on the right, they are added as a complex. C, data from Table 1 obtained with mAb-N₁ and mAb-C₁ under the two experimental conditions shown in B fit to the equations of Perelson and DeLisi. D, dependence of [R]/[R₀], [RL]/[R₀], and [RLR]/[R₀] on AMH complex concentration. In C and D, K₁ and [R₀]/K₂ values were determined by simultaneously fitting all data to the predicted curves and minimizing the RMSE. Error bars show standard deviations. Ab, antibody; AMH, anti-Müllerian hormone; AMHR2, anti-Müllerian hormone receptor type-2; c-AMH, cleaved AMH.