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. 2021 Oct 22;2(1):100066. doi: 10.1016/j.xjidi.2021.100066

Figure 4.

Figure 4

KGF causes keratinocyte hyperproliferation in HEE. HEEs (n = 3) were stimulated with a dosage range of KGF for 72 hours. EdU was incorporated 24 hours before harvesting on day 8 of the air‒liquid interface. (a) H&E, Ki-67, and EdU staining on KGF stimulation and (b) quantification of Ki-67‒ and EdU-positive nuclei/mm length of the HEE. (c) Epidermal thickness (P = 0.008). (d) H&E, Ki-67, and EdU staining and (e) quantification of HEEs pretreated with 100 nM MEK/ERK inhibitor (U0126) for 24 hours and then costimulated with 100 ng/ml KGF for 72 hours (Ki67, P = 0.004; EdU, P = 0.011) (n = 3). ∗P < 0.05. Data are presented as mean ± SEM. Bar = 100 μm. EdU, 5-ethynyl-2′-deoxyuridine; HEE, human epidermal equivalent; KGF, keratinocyte GF; MEK/ERK, MAPK/extracellular signal‒regulated kinase kinase/extracellular signal‒regulated kinase kinase.