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Prolonged HEEs cultures stimulated with IL-4. HEEs were stimulated with 10 ng/ml IL-4 from day 1 of the air‒liquid interface and harvested on days 12, 16, or 20 of culture. H&E and Ki-67 staining show morphological changes and keratinocyte proliferation during long-term epidermal differentiation (n = 1). Bar = 100 μm. HEE, human epidermal equivalent.
