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. 2022 Jan 17;12:815997. doi: 10.3389/fphys.2021.815997

FIGURE 1.

FIGURE 1

In situ hybridization of TPH2 and SERT in the P1 chick brainstems. Digoxigenin-labeled RNA antisense [TPH2 (A–D), and SERT (E–H)] and sense [TPH2 (A’–D’) and SERT (E’–H’)] probes were used for in situ hybridization in P1 chick brain coronal sections. To evaluate the expression patterns of TPH2 and SERT, sections of six chicks for TPH2 and four chicks for SERT were analyzed, and the representative levels of sections (A2.4, A2.0, A1.2, and 1.0) are shown. The levels of the sections are in accordance with those mentioned in Kuenzel and Masson’s chick atlas (Kuenzel and Masson, 1988). (I–L) Diagrams of coronal sections shown in panels (A–D). B2, B2 cell group; B8, B8 cell group; DR, dorsal raphe; FLM, fasciculus longitudinalis medialis; IO, nucleus isthmo-opticus; MR, median raphe; nIV, nucleus nervi trochlearis; OMN, oculomotor nucleus; SCv, nucleus suboeruleus ventralis; P1, post-hatch day 1. Scale bars = 2.5 mm (A–C,A’–C’) and 1 mm (D,D’,H,H’).