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Double in situ hybridization of 5-HTRs and TPH2 in the MR (B6) of the P1 chick brainstems. Digoxigenin-labeled (5-HTRs) and fluorescein-labeled TPH2 RNA antisense (A–E) and sense (A’–E’) probes were used for double in situ hybridization in coronal sections of P1 chick brains. (A”–E”) Diagrams of coronal sections are shown in the rightmost panels. Arrowheads indicate signals. MR, median raphe; P1, post-hatch day 1. Scale bars = 100 μm.
