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The intracellular survival rate of macrophages infected with recombinant M. smegmatis. THP-1 cells were infected with recombinant M. smegmatis MS_Rv2650c and MS_Vec, respectively, and the cells were lysed with SDS at the corresponding time. The cell lysate was diluted and coated on MB 7H10 plate. The number of single colonies was calculated after incubation for 3–4 days at 37°C. Similar results were obtained in 3 independent experiments. (*p < 0.05).
