FIGURE 3.

Structural differentiation between FLC- and MIKC ^* -like MADS-box genes. Amino acid sequences of representative genes from both subfamilies were aligned using Clustal Omega (Sievers et al., 2011) with default parameters and multiple sequence alignment visualized with MEGA7 (Kumar et al., 2016). FLC-like genes (above solid black line) were separated from MIKC ^* -like genes (below solid black line). The conserved and diverged residues were indicated with star (*) and asterisk (#) symbols, respectively. Multiple sequence alignment demonstrated conservation of amino acid residues/motifs within both subfamilies, whereas diversification between subfamilies.