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. 2022 Jan 17;12:818880. doi: 10.3389/fgene.2021.818880

FIGURE 5.

FIGURE 5

Boxplots showing evolution patterns in M- and MIKC-type wheat MADS-box genes. The coding sequences of the duplicated genes were MAFFT aligned (Katoh and Standley, 2013; Katoh et al., 2018), masked and subjected to SNAP V2.1.1 program for computing the synonymous (Ks) and non-synonymous (Ka) substitution rates. The divergence time between duplicated genes pairs was estimated in million years ago (MYA) by following El Baidouri et al. (2017) and boxplots were generated using Microsoft Excel 2019.