Figure 2.

Evaluation of motor behaviors in mice immunized with MOG₃₅₋₅₅ (QuilA-EAE) and their controls (CTL) at post-induction Day 17 (D17) and D30. (A) Evaluation of spontaneous locomotor activity using the open-field test. Locomotion was evaluated using the total distance traveled ± SEM for the overall 15 min session. (B) Rotarod performance was expressed as the mean latency to fall ± SEM for the four rotarod sessions performed by each mouse. (C) Muscular strength performance was expressed as the mean grip strength ± SEM for the five sessions performed by each mouse. Top: forelimb muscular strength. Bottom: fore and hindlimb muscular strength. (D) Evaluation of fine coordination using the grid test. Top: locomotion was evaluated using time to travel the 300 cm required distance ± SEM. Bottom: fine motor coordination was assessed using the number of paw slips on holes in the grid ± SEM for a distance of 300 cm. Statistical analysis was performed using two-way ANOVA followed by post-hoc Sidak test; ^**p < 0.01 vs. control (shown for Sidak test only).