Figure 4.

Evaluation of orofacial sensitivity in mice immunized with MOG₃₅₋₅₅ (QuilA-EAE) and their controls (CTL) at post-induction Day 17 (D17) and D30. (A) Mechanical dynamic sensitivity to smooth and rough paintbrushes. Mechanical dynamic sensitivity expressed as the mean number of positive responses ± SEM induced by rubbing the whisker pad with a smooth paintbrush made of marten hairs (top) or a rough paintbrush made of pig bristles (bottom). (B) Mechanical static sensitivity to three stimuli of calibrated air puffs (duration and intensity): Stimulation 1 (0.16 s, 1.8 mpsi), Stimulation 2 (0.14 s, 23.0 mpsi), Stimulation 3 (0.48 s, 26.4 mpsi). Mechanical static sensitivity expressed as the mean nociceptive score ± SEM induced by each air puff. (C) Cold allodynia expressed as time spent rubbing or scratching face ± SEM for 1 min after acetone drop deposition. Statistical analysis was performed using two-way ANOVA followed by post-hoc Sidak test; ^**p < 0.01 vs. control (shown for Sidak test only).