Figure 5.

Evaluation of cognitive behaviors using the three-chamber sociability and social novelty test in mice immunized with MOG₃₅₋₅₅ (QuilA-EAE) and their controls (CTL) at post-induction Day 30. Left: Schematic representation of the three-chamber sociability and social novelty test. Top: the T1 session when an unfamiliar adult WT female mouse was placed in one of the wire cups for the evaluation of sociability. Bottom: the T2 session where the first intruder is still posted in its wire cup but another unfamiliar adult WT female mouse was placed in the second cup for the evaluation of preference for social novelty. Top right: Sociability evaluated using the sociability index (exploration time of unfamiliar mice – exploration time of empty cup / total exploration time of both cups) ± SEM over the T1 5 min session. Bottom right: Social novelty evaluated using the percentage of novelty [(exploration time of unfamiliar mice/total exploration time of both mice) × 100] ± SEM for the T2 5 min session. Statistical analysis was performed using Student t-test, ^**p < 0.01 vs. control.