Figure 6.

Pharmacological evaluation of pregabalin effects on mechanical sensitivity and heat hyperalgesia in mice immunized with MOG₃₅₋₅₅ (QuilA-EAE) and their controls (CTL) during the chronic phase of the disease. Mice received a single intraperitoneal (i.p.) bolus dose of pregabalin at 10 or 30 mg/kg or vehicle (NaCl sterile for injection). (A) Mechanical sensitivity assessed by the von Frey frequency method using calibrated 1.4 g von Frey hair filaments. Measurements were made before injection and for 2 h after injection and expressed as the mean percentage of paw withdrawal ± SEM. Results are represented as time course curves for each group and as areas under the curve (AUC) calculated for each group and represented by a bar chart. Data for CTL mice are given on the left and data of QuilA-EAE mice on the right. (B) Heat hyperalgesia evaluated by a hotplate test at 56°C. Measurements were made before injection and 2 h after injection and expressed as the mean latency of the first nocifensive behavior ± SEM. Results are represented as a time course curve for each group and as area under the curve (AUC) calculated for each group and represented by a bar chart. Data of CTL mice are given on the left, data of QuilA-EAE mice on the right. For AUC, statistical analysis was performed using one-way ANOVA followed by post-hoc Tukey's test; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.